Acetyltransferase hMof and deacetylase HDAC3 coordinately regulate the levels of hMSH4 acetylation in response to DNA harm induced by IR. Acknowledgments We thank Nianxi Zhao for technical assistance and Thomas Sudhof and Eric Verdin for generously giving the mammalian expression constructs pcDNA3.1-N-HA-hTip60 and pEV-589-HDAC3-Flag. This function was supported in part by NIH Grant GM084353 (C.H.). Conflicts of Interest The authors declare that they’ve no competing interests. References 1. 2. Lee, K.K.; Workman, J.L. Histone acetyltransferase complexes: One size does not fit all. Nat. Rev. Mol. Cell Biol. 2007, 8, 284?95. Choudhary, C.; Kumar, C.; Gnad, F.; Nielsen, M.L.; Rehman, M.; Walther, T.C.; Olsen, J.V.; Mann, M. Lysine acetylation targets protein complexes and co-regulates big cellular functions. Science 2009, 325, 834?40. Gu, W.; Roeder, R.G. Activation of p53 sequence-specific DNA binding by acetylation from the p53 C-terminal domain. Cell 1997, 90, 595?06. Jiang, X.; Sun, Y.; Chen, S.; Roy, K.; Price, B.D. The FATC domains of PIKK proteins are functionally equivalent and take part in the Tip60-dependent activation of DNA-PKcs and ATM. J. Biol. Chem. 2006, 281, 15741?5746. Yang, X.J. The diverse superfamily of lysine acetyltransferases and their roles in leukemia and also other diseases. Nucleic Acids Res. 2004, 32, 959?76. Cohen, H.Y.; Lavu, S.; Bitterman, K.J.; Hekking, B.; Imahiyerobo, T.A.; Miller, C.; Frye, R.Buy1538005-13-8 ; Ploegh, H.; Kessler, B.M.; Sinclair, D.A. Acetylation with the C terminus of Ku70 by CBP and PCAF controls Bax-mediated apoptosis. Mol. Cell 2004, 13, 627?38. Yamaguchi, H.; Woods, N.T.; Piluso, L.G.; Lee, H.H.; Chen, J.; Bhalla, K.N.; Monteiro, A.; Liu, X.; Hung, M.C.; Wang, H.G. p53 acetylation is essential for its transcription-independent proapoptotic functions. J. Biol. Chem. 2009, 284, 11171?1183. Jeong, J.; Juhn, K.; Lee, H.; Kim, S.H.; Min, B.H.; Lee, K.M.; Cho, M.H.; Park, G.Price of 5-Bromo-4-methoxy-2-methylpyridine H.PMID:23927631 ; Lee, K.H. SIRT1 promotes DNA repair activity and deacetylation of Ku70. Exp. Mol. Med. 2007, 39, eight?3.3. four.five. six.7.8.Int. J. Mol. Sci. 2013, 14 9. ten.11.12. 13.14.15.16. 17.18.19.20. 21.22.Yuan, Z.; Zhang, X.; Sengupta, N.; Lane, W.S.; Seto, E. SIRT1 regulates the function of the Nijmegen breakage syndrome protein. Mol. Cell 2007, 27, 149?62. Terui, T.; Murakami, K.; Takimoto, R.; Takahashi, M.; Takada, K.; Murakami, T.; Minami, S.; Matsunaga, T.; Takayama, T.; Kato, J.; Niitsu, Y. Induction of PIG3 and NOXA via acetylation of p53 at 320 and 373 lysine residues as a mechanism for apoptotic cell death by histone deacetylase inhibitors. Cancer Res. 2003, 63, 8948?954. Gupta, A.; Sharma, G.G.; Young, C.S.; Agarwal, M.; Smith, E.R.; Paull, T.T.; Lucchesi, J.C.; Khanna, K.K.; Ludwig, T.; Pandita, T.K. Involvement of human MOF in ATM function. Mol. Cell Biol. 2005, 25, 5292?305. Tang, Y.; Luo, J.; Zhang, W.; Gu, W. Tip60-dependent acetylation of p53 modulates the decision involving cell-cycle arrest and apoptosis. Mol. Cell 2006, 24, 827?39. Jiang, Z.; Kamath, R.; Jin, S.; Balasubramani, M.; Pandita, T.K.; Rajasekaran, B. Tip60-mediated acetylation activates transcription independent apoptotic activity of Abl. Mol. Cancer 2011, 10, 88. Sharma, G.G.; So, S.; Gupta, A.; Kumar, R.; Cayrou, C.; Avvakumov, N.; Bhadra, U.; Pandita, R.K.; Porteus, M.H.; Chen, D.J.; Cote, J.; Pandita, T.K. MOF and histone H4 acetylation at lysine 16 are important for DNA damage response and double-strand break repair. Mol. Cell Biol. 2010, 30, 3582?595. Paquis-Fluck.