Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Cardiovasc Pharmacol. Author manuscript; out there in PMC 2014 April 01.Khan et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure 1. Cardiac function in fish oil (FO) and non-purified diet plan (NPD)-fed control (Ctrl) and MHC-ACS1 (ACS1) miceEchocardiography was utilized to assess cardiac function in 14-week old mice fed a nonpurified diet program (NPD) or diets containing either high (HD) or low dose (LD) FO for six weeks. White bars represent handle mice and black bars represent MHC-ACS1 mice. A: Fractional shortening (FS). n=3?0 mice per group. 2-way ANOVA for FS: genotype P = 0.0028; diet regime P = 0.0008; genotype ?diet program, P = 0.056. a, P 0.001 vs. control NPD-fed mice; b, P 0.05 vs. MHC-ACS1 NPD-fed mice (by student t-test); c, P 0.001 vs. MHC-ACS1 NPD-fed mice; d, P 0.05 vs. MHC-ACS1 LD FO-fed mice. B: Left ventricular diastolic dimension (LVDd) n=3?0 mice per group. Two-way ANOVA for LVDd: genotype P = 0.0025; diet regime PJ Cardiovasc Pharmacol. Author manuscript; obtainable in PMC 2014 April 01.Khan et al.Page= 0.059; genotype ?diet plan, P = 0.665. a, P 0.01 vs. control NPD-fed mice; b, P 0.05 vs. MHC-ACS1 NPD-fed mice (by student t-test); C: Heart weight/body weight (HW/BW) ratio n=4?7 mice per group. Two-way ANOVA for LVDd: genotype P 0.001; diet program P = 0.0234; genotype ?diet plan, P = 0.71. a, P 0.001 vs. control NPD-fed mice; b, P 0.05 vs. MHC-ACS1 NPD-fed mice;, D: Gene expression of BNP was analyzed by real-time PCR and normalized to 18S expression. n=3? mice per group. Two-way ANOVA for BNP mRNA: genotype P = 0.082; diet plan P = 0.062; genotype ?diet, P = 0.048. a, P 0.01 vs. handle NPD-fed mice; b, P 0.Ethyl 2-cyano-2-(hydroxyimino)acetate Price 05 vs. MHC-ACS1 NPD-fed mice; c, P 0.01 vs. MHC-ACS1 NPD-fed mice. E: Survival curve of MHC-ACS1 mice. n = 12?5 mice per group. a, P 0.05 vs. manage NPDfed mice; b, P 0.05 vs. MHC-ACS1 NPD-fed mice.NIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptJ Cardiovasc Pharmacol. Author manuscript; obtainable in PMC 2014 April 01.Khan et al.PageNIH-PA Author Manuscript NIH-PA Author Manuscript NIH-PA Author ManuscriptFigure two. Cardiac fibrosis and inflammationRepresentative slides from the left ventricle of eight and 14 week old manage and MHC-ACS1 mice fed either non-purified diet regime (NPD), purified diet program (PD), LD, or HD FO stained for a: collagen deposition (blue) using Masson’s Trichrome (magnification 200?, n = 3? mice per group, and quantified as fibrosis of total location.Formula of Dde-Dap(Fmoc)-OH 2-way ANOVA for location of fibrosis: genotype P 0.PMID:24238415 0001; diet P 0.0001; age P = 0.2277; genotype ?eating plan = 0.0008; genotype ?age = 0.4525, a, P 0.001 vs. 8-wk old NPD-fed control mice; b, P 0.05 vs. 14-wk old MHC-ACS1 NPD-fed mice, c, P 0.05 vs. PD-fed MHC-ACS1 mice. cc, P 0.01 vs. PD-fed MHC-ACS1 mice. d, P 0.001 vs. LD-fed MHC-ACS1 mice. B: -smooth muscle actin (SMA) expression to assess for fibroblast activation in 14-wk old handle and MHC-ACSJ Cardiovasc Pharmacol. Author manuscript; obtainable in PMC 2014 April 01.Khan et al.Pagemice fed NPD or HD FO (magnification 400?, n = six? mice per group. White and black bars represent control and MHC-ACS1 mice respectively. 2-way ANOVA for -SMA expression: genotype P 0.0001; diet regime P 0.0001; P genotype ?eating plan 0.0001, a, P 0.001 vs. handle NPD-fed mice; b, P 0.001 vs. MHC-ACS1 NPD-fed mice. C: MOMA-2 expression in manage and MHC-ACS1 mice fed NPD or HD FO (magnification 400?, n = 6? mice per group. White and black bars represent manage a.