For four minutes. The centrifuged media had been subjected to SDS-PAGE on a
For 4 minutes. The centrifuged media have been subjected to SDS-PAGE on a 7.five Tris-HCl gel (Bio-Rad Laboratories), with subsequent electroblotting transfer onto a 0.45-mm nitrocellulose membrane (Bio-Radajp.amjpathol.org-The American Journal…