+; MS2:381 (100),363,335; MS3:311,317,293,265,169 499[M+H]+; MS2:337 (100),267,171; MS3:171 (100),154,144,130,118 499[M+H]+; MS2:337 (one hundred),267,171; MS3:171 (100),154,144,130,118 Identification Protocatechuic acid Vanillicacid4ODapiofuranosyl (1″6′)Dglucopyranoside Neochlorogenic acid Logaric acid 3,4dimethoxyphenolDapiofuranosyl (1″6′)Dglucopyranoside Chlorogenic acid Loganin Kelampayoside A Cryptochlorogenic acid Iso-naucleamide A10ODglucopyranoside 7-dehydrated logaric ether Iso-naucleamide A10ODglucopyranoside Sweroside IsonaucleamideA10ODgluco pyranosyl(16)Dglucopyranoside Naucleamide A10ODglucopyranoside Naucleoxoside C Naucleamide G Pumiloside 3-epi-pumiloside 10-hydroxystrictosamide Naucleoxoside A or B Naucleoxoside A or B 35tetrahydrodeoxycordifoline lactam Strictosamide VincosamideHPLCDADESI/MS: Higher performance liquid chromatographydiode array detector electrospray ionisationmass spectrometry, MS: Mass spectrometrT he ESIMS n of the naucleamide A10 O Dglucopyranoside has been nicely investigated.1256245-84-7 Chemscene [29] The fragmentation pathway of [M + H]+ ion m/z 519 was shown in the good mode. Firstly, the [M + H]+ ion produced a prominent ion m/z 357 inside the MS/MS spectrum, which revealed a neutral loss of 10Oglucose residue. After which the obtained ion developed the prominent [M – glu – H2O + H]+ ion at m/z 339. Then the [Mglu – H2O + H]+ ion of m/z 339 was chosen for further MS4 evaluation to create ions at m/z 321, m/z 309 and m/z 160 were also observed, which were attributedThe ESIMSn of naucleamide A10ODglucopyranosideto the losses of H2O, CH4O and RDA cleavage of ring C, respectively.Price of (5-(tert-Butyl)-1H-pyrazol-3-yl)methanol The fragmentation of naucleamide G exhibited a bit difference from that of naucleamide A10ODglucopyranoside because of the CH2OH in the C16 position.PMID:23255394 In the MS/MS spectr um, the [M – glu + H]+ ion at m/z 327 was discovered because the base peak. After which the obtained ion developed the prominent [M – glu – H2O + H]+ ion at m/z 309. Successively, inside the MS four spectrum of m/z 309, two ions at m/z186 and 160 had been observed, which werePharmacognosy Magazine | July-September 2014 | Vol 10 | IssueThe ESIMSn of naucleamide GZhu, et al.: Qualitative and quantitative analysis of constituents in Danmu injectionglu: glucopyranoside; api: apiofuranosyFigure two: The structures of 25 compounds identified in Danmu injectionassigned because the RDA cleavage of ring D and C, respectively [Figure 6].The ESIMSn of pumiloside and its isomer 3epipumilosidePumiloside and 3epi pumiloside, with the exact same MS spectra, UV, molecular formulae, are isomers. Pumiloside and its isomer 3epi pumiloside would be the only two quinoline alkaloids in Nauclea officinalis,[2426] and pumiloside is larger content in Danmu injection. ThePharmacognosy Magazine | July-September 2014 | Vol 10 | IssueESIMSn with the pumiloside and 3epi pumiloside had been studied.[29,30] In their ESIMS spectra, the [M + H]+ ion was observed, when the significant [M – glu + H]+ ion was found inside the MS/MS spectrum. The [M – glu + H]+ ion was triggered by the loss of H2O and CO2 in the MS3 spectrum, in the same time, the ion at m/z 281 brought on by the loss of neutral fragments C 4H 6O four of RDA cleavage of ring E could possibly be observed in the MS3 spectrum. Then the ion m/z 281 was selected for further MS4 evaluation to produce two ions at m/z 185and 158,Zhu, et al.: Qualitative and quantitative analysis of constituents in Danmu injectionFigure 4: MS/MS spectrum of swerosideand then [MgluCO2+H]+ ion m/z 335 was observed inside the MS3 spectrum, successively.